DuPont™ AmberLite™ XAD™ 4 Polymeric Adsorbent Resins

DuPont™ AmberLite™ XAD™ polymeric adsorbent resins are nonionic, macroporous crosslinked resins that selectively adsorb molecules based on a combination of hydrophobic interactions and molecular size.  These resins can be used in single-use adsorption mode to capture impurities from products streams for disposal, or they can be regenerated and used in an adsorption/desorption mode to separate products and impurities across many production batches.

DuPont™ AmberLite™ XAD™ 4 is a crosslinked divinylbenzene (DVB) resin with water retention capacity of 54-60%, average surface area of 750 m2/g, and a mean pore size of ~100 Å.  See Product Data Sheet for more information.

DuPont offers a range of XAD™ products of varying chemistries, particle sizes, surface areas, average porosities, and pore sizes.  Products are sold as free-flowing wet resin beads.  The main product characteristics to consider when selecting an adsorbent resin for bioprocessing are the following:

but will have higher backpressures.
• Surface Area:
Surface area impacts operational capacity and kinetics, with higher surface area giving
higher operational capacity and faster kinetics.
• Porosity:
Porosity is related to the surface area. Particles with high porosity will have more surface area
for the molecules to interact with, provided the pore size is large enough to accommodate
the molecule. Therefore, higher porosity will generally lead to higher operational capacity.
• Pore Diameter:
Pore diameter impacts the degree of separation and molecule recovery.
Large pores will allow better recovery of large molecules.
• Chemistry:
The chemistry of the resin (DVB vs acrylic) impacts how tightly molecules are held.
Hydrophobic molecules will be more loosely held on acrylic particles, making it easier to
desorb the bound molecules and reducing the amount of solvent required in the desorption step.

Product Data Sheet

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